2.7 å Thank you On Mar 26, 2012, at 1:26 PM, Ed Pozharski wrote:
> And the resolution of your data is... ? > > On Mon, 2012-03-26 at 19:58 +0100, Alok Shenoy wrote: >> Hello, >> >> I'm working on a structure using coot, and I have a peculiar problem. >> Searching www didn't quite provide me with an answer, hence requesting more >> knowledgeable users for some help. >> >> Background: >> The structure has 2 chains, Chain A and Chain B. Chain B has all the >> residues that should be present according to our sequence, and the numbering >> on both chains are complete. >> >> Problem: >> 1) Chain A has a couple of residues missing. The numbering shows 231 232 >> 234. Clearly marking out the spot where the residue is missing. Sequence >> shows that there is an aspartic acid missing in position 233. Problem is - >> both 232 and 234 are connected quite normally, inserting a terminal residue >> at 232 (alanine at first) results in absolutely weird connections. Deleting >> specific atoms in the backbone on the protein at that position (between 232 >> and 234) don't quite help either - as that results in 234 missing a peptide >> bond. >> >> 2) At some points, there is no backbone -by which I mean - lets say residue >> 100 and 101 side chains are showing up (and have appropriate electron >> density). What is missing is the peptide bond that connects these two >> residues (again - electron density for the backbone is present). Is there a >> way to fix this? >> >> Can someone walk me through the solution to this problems? >> >> >> Thank You, >> >> Alok Shenoy > > -- > Oh, suddenly throwing a giraffe into a volcano to make water is crazy? > Julian, King of Lemurs >
