Hi Michael, How did you add the phosphorylated residue? Did you use 'Extensions -> Modelling -> Phosphorylate this residue'? If so the problem may be, that the chain ID and residue number of that residue are changed. If you change that back to what it was, I will probably work.
Hope that helps, Bernhard Bernhard C. Lechtenberg, PhD Postdoctoral Associate Riedl Lab Sanford-Burnham Medical Research Institute 10901 North Torrey Pines Road La Jolla, CA 92037, USA Phone: 858.646.3100 x 4216 Email: [email protected] From: Mailing list for users of COOT Crystallographic Software [mailto:[email protected]] On Behalf Of Gmail Sent: 04 February 2013 16:58 To: [email protected] Subject: phospho-serine or any phosporylated residue Dear Coot users, Has anyone had trouble with fitting the main chain for phosphorylated residues in coot? After i do real space Refine Zone over the phosphorylated residue the main-chain of the phosphylated-residue is not connected to the adjacent residues. Is there some trick to getting the main-chain connected? My density maps are ~2.5A resolution and the residue has the standard name such as HETATM PTR. thanks for helping with useful suggestions. Michael
