Hi Patrick, If you have a pdb file and a corresponding mtz file, coot can do it for you. You read in the mtz file (File -> Open MTZ, ...) and it will ask you which PDB file to use for calculating phases for the maps.
If you download the data from pdbe.org you get a *.ent file which you can convert to mtz with cif2mtz (heres an example with 2xn2): cif2mtz hklin r2xn2sf.ent hklout 2xn2.mtz (then write END at the prompt) This should get you going, Best regards, Folmer PS: I just saw Pete's answer, and if we are speaking strictly about the PDB, and if it's permitted to use phenix, then phenix.fetch_pdb is easier to deal, I think: phenix.fetch_pdb 2xn2 --maps PPS: OK, now Nat already mentioned this... but here it comes anyway :-) 2014-05-27 22:42 GMT+02:00 RiC <[email protected]>: > HI, > > Does anyone have a step by step guide to go from a structure factor file > from the PDB to a CCP4 map (understandable to a biologist) like the one I > can download on the electron density server? > > I am confused and my attempts at using CCP4 keeps coming up with "failed" > > Thanks > Patrick > > ____________________________________________________________ > FREE ONLINE PHOTOSHARING - Share your photos online with your friends and > family! > Visit http://www.inbox.com/photosharing to find out more! > -- Folmer Fredslund
