I have a situation where I manually edited my PDB file to rename MET to MSE, 
then made the appropriate change to change all 
“ SD" atoms in MSE to “SE “.  When I optimize the geometry with Real Space 
Refine Zone, the amide proton (H) moves several Å way, and the Torsions error 
goes to 2.24.

Do you have any idea what’s causing this?
_________________________________
Mark A. Saper, Ph.D.                            
Associate Professor of Biological Chemistry, University of Michigan
Ann Arbor MI  48109-1055 U.S.A.
[email protected]     phone (734) 764-3353    fax (734) 763-4581

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