Hi,
I'm trying to model two states in a ligand-substrate-enzyme complex. That is, there is density that suggests that the substrate is partially linked to the enzyme and also, but independently, linked to the inhibitor. I've tried building both the enzyme-substrate covalent conjugate and the inhibitor-substrate covalent conjugate and overlaying them on top of each other, but this hasn't worked. I usually use the alternate conformations tool for issues like this, but since these are actually two distinct chemical entities that occupy the same space (I'm currently assuming they split the occupancy 50:50), I couldn't determine how to model them both simultaneously without significant clashes. Best, Sam
