Dear Paul, this works. Of course I also had to save the modified mmCIF file of ACE and then include it in the refmac refinement.
Thanks a lot. Stefan Am Jun 9, 2017 um 5:41 AM schrieb Paul Emsley <[email protected]>: > On 08/06/2017 03:26, Becker, Stefan wrote: >> Hi all, >> in the coot mail archive I found an instruction from 2013 for N-terminal >> acetylation: build ala, replace by ACE, edit restraints for ACE to group >> L-peptide and apply. The last step does not work, changing to L-peptide >> cannot be applied. Could someone give me advice about this? Thanks in >> advance. > > I would try > > File -> Get Monomer -> ACE -> OK > Edit Restraints -> ACE -> OK -> Info -> Group to "L-peptide" -> {Return} -> > Apply > Add Terminal Residue (to current N-terminal protein molecule) > Replace Residue (newly added ALA) to ACE > > Paul.
