Dear all,
this is my first thread here, and I am not sure this topic was discussed or not, please tell me if it was. Coot I thought is originally for density map of crystal, it can work on the cryo-em map too. I have a cryo-em map of helical filament and try to build a model. I can build a model based on the monomer, but I am not sure how to generate the copies based on the symmetry. I think the key problem is how to determine the rotation center which based on the map, then the roation center together with the known helical parameters can do it. suppose we got the rotation center, how to implement these operations in Coot? Certainly, there is a stuipd way which I already did, that is to copy the refined monomer multiple times and fit them all together, later were refined using the Phenix. another way is try to edit the pdb file directly. rotate and tranlate the monomer, later change the chain ID. But I want to do it simplely in Coot, could it possible? Best Wishes, Yao-Wang Li School of Life Science, Tsinghua University Room 255, New Biology Building, Beijing, China, 100084 ######################################################################## To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=COOT&A=1
