Hi Ahmad What happens if you center on your protein? My best guess would be that your map has some dummy symmetry so you get a lot of unit cells visible when you zoom out.
Best regards Folmer ons. 20. mar. 2019 20.49 skrev Ahmad Khalifa <[email protected]>: > I have a cryo-EM map that I want to segment. I have structures surrounding > the density of interest from all sides. I used chimera to subtract > everything and clean the density using volume eraser. > > The screenshot is what I got: > > 1) the structure is not fitted > 2) the density appears to have multiple copies of my proteins, it's > supposed to be only one there! > > What is wrong here exactly, I'm suspecting it's the volume eraser! Is > there a better way to segment the density given how I have atomic models > for all the surrounding densities, but not the density of interest? > > Regards. > > ------------------------------ > > To unsubscribe from the COOT list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=COOT&A=1 > ######################################################################## To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=COOT&A=1
