Hi all, I'm sure this has been asked, but after some searching I can't find hints. Apologies if redundant, but can someone refresh me on how to get Coot to be graceful about residues with insertion codes...particularly during real space refinement. I have a protease structure with very good resolution/density but some odd numbering conventions with insertion code (e.g. 124A, 124B, 124C between residues 124 and 125 numbering) in other areas continuous residues have non-contiguous numbering. When doing real space refinement both situations tend to blow apart, each individual insertion code-numbered residue becoming its own disconnected amino acid and well out of the density, I assume due to sterics and it not interpreting these as being connected. Also common in Fab structures.
I know there are painful workarounds to number it contiguously without insertions and then add back when done refining (but then you find one more thing...), but I am aspiring to greater elegance these days. Also hoping I don't have to define with LINK records or such for each case? Thanks, Seth ######################################################################## To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=COOT&A=1
