Morning, Can anyone suggest the correct method for introducing a non natural amino acid into a protein backbone? I am aware of the modelling, replace residue option but in this instance I am having no joy in creating a protein chain that can subsequently be real space refined etc.
The residue in question is a Trp with NE1 substituted for S 3-(1-benzothiophen-3-yl)-L-alanine The issue is not that I am unable to introduce the change but I have not thus far ended up with a protein that is correctly bonded. Changing residue numbers and chain labels does allow for peptide bond formation but it does not look quite right and any subsequent refinement in COOT blows the chain apart. I am working with COOT 0.9-pre but also have access to 0.8.9. Many thanks for any pointers, Colin [cid:[email protected]] Dr. Colin W. Levy MIB G016 Tel. 0161 275 5090 Mob.07786 197 554 [email protected]<mailto:[email protected]> [cid:[email protected]][cid:[email protected]][cid:[email protected]][cid:[email protected]][cid:[email protected]] ######################################################################## To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=COOT&A=1
