Hi Paul,

congratulation for the cover of Nature - beautiful pictures!

Do you know why the hydrogen atoms only show up in the difference map,
but not in the direct map? The signal strength of hydrogens relative to
C, N, O is quite strong, much stronger than for X-rays. The large blobs
that you illustrate in the difference map just disappear in the direct
map. Is this expected? After the hydrogens are modelled, they seem to
totally disappear...

Cheers,
Tim


On Thu, 5 Nov 2020 04:44:49 +0000 Paul Emsley
<[email protected]> wrote:

> Hi,
> 
> My colleagues recently published a paper (Nakane et al. (2020)
> "Single Particle cryo-EM at atomic resolution") and asked me to make
> a figure. I did so and it is this week's front cover image.
> 
> https://www.nature.com/nature/volumes/587/issues/7832
> 
> I wrote a short blog post with high resolution images:
> 
> https://pemsley.github.io/coot/blog/2020/11/05/nature-images.html
> 
> Paul.
> 
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-- 
--
Tim Gruene
Head of the Centre for X-ray Structure Analysis
Faculty of Chemistry
University of Vienna

Phone: +43-1-4277-70202

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