On 23/01/2021 17:36, Ahmad Khalifa wrote:
Hi,
I followed this tutorial to add a NAG to my structure
http://legacy.ccp4.ac.uk/tutorials/tutorial_files/jligand/1_glycan.html
<http://legacy.ccp4.ac.uk/tutorials/tutorial_files/jligand/1_glycan.html>.
My question is, how can I add another NAG to make a NAG chain?
This is the wrong question.
You googled "How to build N-linked glycans in Coot" and that was the most pertinent documentation that you
could find? That's a disappointment. Have another go, adding my name and Joosten or Crispin.
Anyway, try this:
Calculate -> Modules -> Carbohydrate
Then you have 2 choices, automatic and semi-automatic:
Glyco -> Add XYZ (e.g. Add High Mannose)
or
Glyco -> N-linked Glycan Addition...
From time to time you might need "Update for Current residue" - depends on how
you move around in Coot.
0.9.4 has adjusted weights, which may make things easier.
Paul.
########################################################################
To unsubscribe from the COOT list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=COOT&A=1
This message was issued to members of www.jiscmail.ac.uk/COOT, a mailing list
hosted by www.jiscmail.ac.uk, terms & conditions are available at
https://www.jiscmail.ac.uk/policyandsecurity/
