Sorry for the delay.

Better late than never, perhaps?


On 12/01/2022 23:04, Georg Mlynek wrote:


With the help of ccp4 people I created link records to build a large ligand from smaller monomers.

One link is FTT-MYR. Cif file attached.

I also put this record in the pdb file

LINKR        O3  FTT E   2                 C1  MYR E 3                FTT-MYR

and did

Import CIF dictionary in coot with the FTT-MYR.cif

However doing sphere_refine the restraints seem to be not applied, because the residues are repelled.


I attach my test molecule. I read in your dictionary and did a regularization - it seemed to work fine. (I didn't use sphere refine because I didn't have density).

I am using Coot 0.9.7. Why don't you say what you are using?



What am I doing wrong?


Why do I have to import the cif file via File --> Import CIF dictionary in coot and can't just past the cif files in C:\CCP4-7\7.1\Lib\data\monomers\list ?


Does this link exist in mon_lib_list.cif?

Coot reads mon_lib_list.cif as it starts up.


Paul.


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