I agree with Eleanor. Based on the plot you sent I don't see a problem. You don't have any outrageous residues, only a few slightly over the line.

Dale Tronrud

On 4/14/2025 4:11 AM, Eleanor Dodson wrote:
Possibly not all residues SHOULD be in the most favoured regions - local contacts and crystal contacts can distort geometry ..
Eleanor

On Mon, 14 Apr 2025 at 11:59, 张馨丹 <2022324060...@stu.scu.edu.cn <mailto:2022324060...@stu.scu.edu.cn>> wrote:

    Dear Coot Subscribers Forum

         I hope this message finds you well.

         I am currently working on refining a protein structure using
    Coot and aim to achieve 100% residues in the "In Favoured
    Regions" of the Ramachandran Plot.  While there is no outlier, some
    residues still fall outside these regions.I can't find a way to
    increase the percentage of "favoured" any further. I hope to get
    your help.

         Thank you!







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