Hi Marcin, CryoSPARC, RELION, cisTEM, ChimeraX, IMOD, Phenix, MOE and (I assume) some other packages handle mode 12.
There must be files available that you could download. Or just convert any MRC file you already have with IMOD (off the top of my head: newstack -mode 12 old.mrc new.mrc). Cheers, Alexis — Alexis Rohou Distinguished Scientist Director of CryoEM Genentech, Department of Structural Biology 1 DNA Way South San Francisco, CA 94080 (cell) 781 249 9629 Sent from a mobile device, with apologies for brevity, typos, swypos, and predictive text errors. On Mon, Nov 3, 2025 at 4:47 AM Marcin Wojdyr <[email protected]> wrote: > Hi Alexis, > That's a relatively new mode. I don't know any software that handles > it. Are there any publically available examples of such files? > > Marcin > > On Fri, Oct 31, 2025 at 8:24 PM Alexis Rohou > <[email protected]> wrote: > > > > Hi Paul, > > > > Unless I'm mistaken, coot doesn't handle MRC maps in mode 12 (16-bit > floats) correctly. At least with coot-1.1.19 I couldn't get such a map to > display, but converting it to mode 2 (32-bit floats) and loading the > converted map works just as expected. > > > > If that's all correct, I would humbly request that support be added for > mode 12 MRC maps, which I work with very frequently. > > > > Cheers, > > Alexis > > > > -- > > Alexis Rohou > > Distinguished Scientist > > Director of CryoEM > > Genentech, Department of Structural Biology > > 1 DNA Way > > South San Francisco, CA 94080 > > Cell: 781 249 9629 > > Pronouns: he/him/his > > > > ________________________________ > > > > To unsubscribe from the COOT list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=COOT&A=1 > ######################################################################## To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=COOT&A=1 This message was issued to members of www.jiscmail.ac.uk/COOT, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
