On 01/07/2026 15:35, Pranav Shah wrote:
Hi Paul,
Newb Q for you: Is there a way in COOT to perform flexible fitting
with NCS imposed? My molecule has D4 symmetry and the cryoem map was
produced with D4 symmetry imposed.
If the D4 symmetry is not a consideration, then have a look at the "Coot
with RNA" video on YouTube.
However, one cannot as yet, impose NCS (or molecular symmetry)
restraints during refinement - if that's what you mean.
Regards,
Paul.
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