Hi, I've been experimenting with Ray (hopefully the Ray Meta component but also after digital normalization) for performing some pooled BAC sequencing experiments where the amplicons are often present in highly variable relative copy number. I'd like to perform some manual assessment of the assemblies, but it appears that the individual read names have been replaced with numerical labels in the AMOS file that Ray produces. This makes it impossible to investigate read pair consistency in the assembly. Is there a way to relate the new read names with the original ones that were passed to Ray, or alternatively, are the reads renamed during loading in a systematic way so that I can alter the resulting AMOS file before loading into hawkeye?
Thanks, Anthony Anthony Borneman Principal Research Scientist - Molecular Biology | The Australian Wine Research Institute Waite Precinct, Hartley Grove cnr Paratoo Road, Urrbrae (Adelaide) SA 5064 | Map PO Box 197, Glen Osmond SA 5064, Australia T: +61 8 83136613 (direct) | F: +61 8 83136601 | www: www.awri.com.au | AWRI Events This communication, including attachments, is intended only for the addressee(s) and contains information which might be confidential and/or the copyright of The Australian Wine Research Institute (AWRI) or a third party. If you are not the intended recipient of this communication please immediately delete and destroy all copies and contact the sender. If you are the intended recipient of this communication you should not copy, disclose or distribute any of the information contained herein without the consent of the AWRI and the sender. Any views expressed in this communication are those of the individual sender except where the sender specifically states them to be the views of the AWRI. No representation is made that this communication, including attachments, is free of viruses. Virus scanning is recommended and is the responsibility of the recipient. ------------------------------------------------------------------------------ Everyone hates slow websites. So do we. Make your web apps faster with AppDynamics Download AppDynamics Lite for free today: http://p.sf.net/sfu/appdyn_d2d_nov _______________________________________________ Denovoassembler-users mailing list Denovoassembler-users@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/denovoassembler-users
