On 04/09/13 02:32 AM, Hornung, Bastian wrote:
> Thanks for the answer :).
>
> Why has the download then not been updated with the patch o_O?

Stable releases are thoroughly tested before being distributed.

The next stable release (version 2.3.0) will include these and other 
improvements as well.

> Will applying this patch invalidate the other patch for the PacBio data, 
> which I've applied, or is that one also included in the mentioned link?

You need to apply each patch one after the other. That should work.




>
> Bastian
> ________________________________________
> From: Sébastien Boisvert [sebastien.boisver...@ulaval.ca]
> Sent: Tuesday, September 03, 2013 4:33 PM
> To: denovoassembler-users@lists.sourceforge.net
> Subject: Re: [Denovoassembler-users] Critical exception: The system is out of 
> memory, returned NULL. Requested -168128560 bytes of type 
> RAY_MALLOC_TYPE_BLOOM_FILTER
>
> On 02/09/13 03:57 AM, Hornung, Bastian wrote:
>> Hi @ all,
>>
>> guess I have again a sort of technical problem.
>> I get the error message in the subject line when I try to run my current 
>> assembly (after roughly 10 minutes).
>> I've first run an assembly with ray, with 3 datasets and a kmer size of 33.
>> Now, I'm again doing the same, but I load additionally a set of PCR 
>> fragments via -s in the assembly (30 fragments, shortest 112 bp, longest 
>> ~1.6 kb), and the assembly begins to crash.
>> Same happens when I try to load some FLX data into it (obviously more data), 
>> but that one works on smaller kmer size (only tested with 21, currently it's 
>> running with 27).
>> The machine, on which the assembly is running, has in total 256 GB RAM, and 
>> currently there's more than 200 of it free, and there's also more than 
>> enough hard disk space (80 GB in the home partition, 800 in the working 
>> partition), so I can't see why it should fail at allocating memory :/.
>> Any idea what could be going on?
>>
>
> Hello,
>
> This is a known bug in Ray 2.2.0.
>
> The problem was fixed 5 months ago: 
> https://github.com/sebhtml/ray/commit/3bf06597ed21c6d72cf172884aa1708f37828347
>
>
> You can apply this patch on Ray 2.2.0 with these commands:
>
>
> $ wget 
> http://sourceforge.net/projects/denovoassembler/files/Ray-v2.2.0.tar.bz2
> $ tar -xjf Ray-v2.2.0.tar.bz2
> $ cd Ray-v2.2.0/
> $ wget 
> https://github.com/sebhtml/ray/commit/3bf06597ed21c6d72cf172884aa1708f37828347.patch
>
> $ patch -p1 < 3bf06597ed21c6d72cf172884aa1708f37828347.patch
>
> # apply other patches, if any
>
>
> After that, you can build Ray as usual.
>
>
> Sébastien
>
>> Best regards,
>>
>> Bastian
>>
>>
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