> ________________________________________
> De : Marco van het Hoog [ma...@vanhethoog.com]
> Date d'envoi : 20 janvier 2015 19:50
> À : Sébastien Boisvert
> Objet : Ray assembly of Hamster genome.
> Hello Sébastien ...

Hi,

> I am working for the National Research Council, Biotechnology Research
> Institute in Montreal, and therefore (like you) I have access to all the
> Calcul Quebec servers, including Colosse and Guillimin.

I don't have to these resources now because I am currently working in the U.S.

> We just received 3 HiSeq lanes of reads to construct a genome assembly
> of a Hamster CHO cell line.
> The estimated genome size of Hamster is about 2.8 GB, for Human (if I
> remember well) it's about 3.3 GB, so it's a similar size.
> The 3 lane coverage should be around 30X.
> Could you tell me, if you were to start such a project with Ray, what
> kind of settings you would use?

Take a look at the Ray job scripts that were used for the Assemblathon 2:

https://github.com/sebhtml/assemblathon-2-ray

> Would you use Colosse and Guillimin with 30 cores each, or would you use
> Mammouth Parallèle with 256 or 512 GB in memory?

You would want to use many machines with something like 24 GB RAM each.

> The memory requirements of Ray are a bit confusing to me :)
> Thanks in advance for any suggestion.
> - Marco.
> 

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