Hi,

when you compare the yields based on fresh mass, are you sure you're talking about the same "dung"?

Here in Central Europe cattle often are kept in stables leading to liquid (~8%TS) manure.
In India perhaps "dung" is "dried dung"?
Generally, for comapring gas yields of substrates it's better to compare gas yields based on VS, not fresh matter, since water content may vary a lot.

I can't think that there's a production of 18 times more biogas if we are talking about comparable dung. You may calculate/estimate a C-Balance. If there's one loading, you can't get more C in CH4/CO2 out of the system than you have put into it with the substrate/inoculum in the beginning.

Markus Schlattmann




Am 24.10.2010 11:31, schrieb Anand Karve:
Dear Mr. Bapat,
the biogas plant in Wardha, which accepts 1000 kg cattle dung as a one-time load and produces daily 3 cubic meter biogas continuously over a period of 180 days, was an absolutely novel system to me. In fact that is why I reported it, because I felt that somebody in the AD discussion group maight know more about it. Since neither the British scientists nor any of the Indian scientists present there could give a scientific explanation to this phenomenon, I have ventured a plausible explanation. The Archaea are a very ancient group of organisms. Lignin is produced by green plants, which evolved much later. Therefore the methanogens cannot digest lignin. The fact that in Wardha, this particular biogas plant was producing almost 18 times as much biogas as would be expected, can be explained by the assumption that lignin was being digested by some other organisms and the products of the lignin digesting organisms were being made available to the mehanogens. But the speculation that some species of organisms conduct extra-cellular digestion of cellulose or lignin, and make the products of such digestion available to the methanogens, is not acceptable to me, because if such were really the case, one would have used such organisms to produce sugars from lignocellulosic material and then obtained alcohol from these sugars. Since nobody has succeeded in doing this, I feel that the organisms that digest cellulose or lignin consume the sugars themselves and multiply their own numbers, and that the methanogens consume these microbes to produce methane. I am ready to accept any other explanation, if it is logical.
Yours
A.D.Karve

On Sun, Oct 24, 2010 at 4:37 PM, Sumedh Bapat <[email protected] <mailto:[email protected]>> wrote:

    Dear Dr. Karve,
    I am sorry to comment on this again but I cannot overlook the
    discrepancy in the information you have provided here..
    */ on October 17 you said : /*
    "In any case, once it is accepted that the methanogenic organisms
    do not digest the dung directly and that they need the help of
    other organisms to digest it, one cannot accept that dung is the
    food of the methanogens. It is like saying that manure applied to
    a field is human food, because through a number of biological
    processes it ultimately ends up into products, which the humans eat."
    */on October 24 you said : /*
    "According to text book knowledge, 1000 kg dung should have
    produced about 30,000 litres (or 30 cubic meters) biogas. But this
    particular biogas plant produces 540 cubic meters of it."

    I also happen to notice that both the subjects refer to cow dung.
    Now it can be seen that you claim that some other plant is
    generating 540 times more gas than your plants.
    Do you mean that this 540 m3 gas that you saw, is produced by
    Methanogens which have consumed other similar organisms from the
    biogas plant , which in turn had "eaten" the Cpw Dung ?
    Can you please explain the sudden Biogas Generation manifold
    increase from 30 m3 (conventionally known) to 540 m3 ?
    /Again/ _ Can you please provide a basis for such a finding ?
    Kind Regards,
    Sumedh Bapat

    On Sun, Oct 24, 2010 at 12:30 AM,
    <[email protected]
    <mailto:[email protected]>> wrote:

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          1. Re: Attachment to previous Article - More scientific based
             research and questions (Anand Karve)


        ----------------------------------------------------------------------

        Message: 1
        Date: Sun, 24 Oct 2010 02:43:13 +0800
        From: Anand Karve <[email protected] <mailto:[email protected]>>
        To: For Discussion of Anaerobic Digestion
        <[email protected]
        <mailto:[email protected]>>
        Subject: Re: [Digestion] Attachment to previous Article - More
               scientific based research and questions
        Message-ID:
        <[email protected]
        <http://mail.gmail.com/>>
        Content-Type: text/plain; charset="iso-8859-1"

        Dear Dr. Martin,
        I have just returned from a city called Wardha, where I
        attended a workshop
        arranged jointly by the Research Councils of UK and the
        Department of
        Science and Technology, Government of India. About 20
        scientists each from
        UK and India were invited to this meeting.
        In the course of field visits organised during the workshop,
        Dr. Soham
        Pandya, The Director of Centre of Science for Villages, an NGO
        in Wardha,
        showed us an amazing biogas plant on his campus. This biogas
        plant accepts
        about 1000 kg cattle dung as a one-time load and produces
        daily about 3
        cubic meters of biogas, continuously over a period of about
        180 days.  This
        is not the only biogas plant constructed by him. Using funds
        from the
        Department of Science and Technology, He has constructed a
        similar biogas
        plant in another place called Hingoli, where a one-time load
         of 1000 kg
        dung yields biogas continuously for 6 months, to run an
        electricity
        generator for daily 3 to 4 hours, to provide electric lighting
        to all the
        houses in the village.  Officials of the Department of Science and
        Technology vouched for the veracity of these claims. According
        to text book
        knowledge, 1000 kg dung should have produced about 30,000
        litres (or 30
        cubic meters) biogas. But this particular biogas plant
        produces 540 cubic
        meters of it.
        Neither Dr. Pandya nor any other scientist could give a
        scientific explanation to this phenomenally high yield of
        biogas. Dung of
        Indian cattle consists mainly of lignin (from the veins and
        midribs of the
        grass and leaves that they feed on) and micro-organisms. One
        has to assume
        in this case, that there are microbes in the dung that feed on
        the lignin
        and that the methanogens digested the lignin eating microbes.
        Yours
        A.D.Karve

        On Mon, Oct 18, 2010 at 3:53 PM, Duncan Martin
        <[email protected] <mailto:[email protected]>>wrote:

        > Perhaps Dr Karve & I should agree to disagree?
        >
        > To argue that dung is not food for the methanogens because
        they need help
        > to digest it is really a semantic quibble. It misses the
        point I was
        > responding to - that the digestion process is not *completed
        *by the act
        > of defaecation, it is merely *terminated* for the owner of
        the gut in
        > question.
        >
        > I have never seen any serious literature suggesting that
        microbes are
        > altruistic. However, the principles of commensalism are well
        established and
        > I see no basis for dismissing them. Moreover, the complex
        web of metabolic
        > interactions in AD has been extensively researched and is
        pretty well
        > understood - though I am sure there is more to discover.
        >
        > Nor have I seen any literature whatsoever suggesting that
        the methanogens
        > consume other microorganisms. I would be intrigued to see a
        proposed
        > mechanism.
        >
        > To dismiss all the textbooks as wrong (see previous
        postings) is unhelpful,
        > at best. Who could only say that unless he had read every
        one of them? Of
        > course, there are mistakes - even in the best books - if
        only because
        > science moves on, so any book becomes outdated. And there
        are indeed some
        > layman's guides to AD that include some odd ideas - but who
        would take them
        > as serious guides to the science?
        >
        > When we find such errors, let us use this forum to report
        them - giving
        > exact references. But lets not confuse newcomers to the
        field by dismissing
        > every other source of information as rubbish.
        >
        > Finally, let us accept that each of us is entitled to his
        opinion - but
        > lets reserve this forum for the fruits of practical
        experience and
        > evidence-based information.
        >
        > I suggest we draw a line under the present debate.
        >
        > Duncan Martin, PhD, MCIWM, MIChemE, MIEI
        > Cloughjordan Ecovillage
        > Ireland
        >
        > On 17 October 2010 16:39, Anand Karve <[email protected]
        <mailto:[email protected]>> wrote:
        >
        >> Dear Duncan,
        >> I dont believe in the theory of a chain of micro-organisms,
        with one
        >> species converting the cellulose into glucose, another
        converting the
        >> glucose into an organic acid (e.g. citric acid), still
        another converting
        >> the organic acid into acetic acid and ultimately the acetic
        acid being
        >> converted by the methanogenic organisms into carbon dioxide
        and methane. If
        >> this were true, one would have by now isolated the organism
        that converted
        >> cellulose into glucose and used the glucose to produce
        alcohol. Cellulose is
        >> the most ubiquitously found organic compount in the world
        and with this
        >> simple process, one would have produced unlimited quantity
        of liquid fuel.
        >> But even today, the conversion of cellulose into glucose is
        achieved in any
        >> industrial process by using a cellulolytic enzyme extracted
        from a
        >> cellulolytic organism. The reason for this is, that the
        glucose converted by
        >> the organism from cellulose is consumed by the same
        organism. And once it is
        >> consumed by an organism, it is converted into its cell all
        the way down to
        >> carbon dioxide. The micro-organisms in the gut of an animal
        cannot be
        >> expected to be so altruistic as to predigest the food and
        suply it to the
        >> methanogens. I feel that the methanogenic organisms consume
        the fellow
        >> micro-organisms in the gut of animals and digest them to
        produce methane and
        >> carbon dioxide. Such dog-eat-dog reactions occur also in
        the soil supplied
        >> with organic matter.
        >>         In any case, once it is accepted that the
        methanogenic organisms
        >> do not digest the dung directly and that they need the help
        of other
        >> organisms to digest it, one cannot accept that dung is the
        food of the
        >> methanogens. It is like saying that manure applied to a
        field is human food,
        >> because through a number of biological processes it
        ultimately ends up into
        >> products, which the humans eat.
        >> Yours
        >> A.D.Karve
        >>   On Fri, Oct 15, 2010 at 4:27 PM, Duncan Martin
        <[email protected] <mailto:[email protected]>
        >> > wrote:
        >>
        >>>
        >>> Yes, the gut methanogens do, in a sense, eat what the
        animal eats.
        >>> However, it would be more accurate to say that their diet
        is derived from
        >>> what the animal eats. The methanogens in the gut of a cow
        are surrounded by
        >>> celluose and other biopolymers but they cannot digest
        them. They live on the
        >>> waste products of other microbial processes. The web of
        metabolic
        >>> interactions is well known.
        >>>
        >>> Where I would "hoot out" Dr Karve is his belief that dung
        cannot serve as
        >>> food for the methanogens because they are "thrown out" of
        the body along
        >>> with the dung. I don't understand the logic here.
        >>>
        >>>
        >>>
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        >> for more information about digestion, see
        >> Beginner's Guide to Biogas
        >> http://www.adelaide.edu.au/biogas/
        >> and the Biogas Wiki http://biogas.wikispaces.com/
        >>
        >>
        >>
        >
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        > http://www.adelaide.edu.au/biogas/
        > and the Biogas Wiki http://biogas.wikispaces.com/
        >
        >
        >


        --
        ***
        Dr. A.D. Karve
        President, Appropriate Rural Technology Institute (ARTI)

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        End of Digestion Digest, Vol 2, Issue 40
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--
***
Dr. A.D. Karve
President, Appropriate Rural Technology Institute (ARTI)

*Please change my email address in your records to: [email protected] <mailto:[email protected]> *




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--

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