Bonnie,

I am not sure you quite understand rarefaction. First, there are 
individual-based and sample-
based versions. I'm guessing you mean individual based, as sample-based version 
also take inter-
sample (i.e., usually spatial) heterogeneity into account.

Individual-based rarefaction simply tells you the number of species you would 
expect to get if you 
took a real sample and extracted a smaller subsample at random. It is used in 
the context of 
uneven sample sizes, normally as a way to to standardize species RICHNESS 
estimates to a 
constant sample size. For example, if you have samples of 100, 150 and 200 
individuals from 
each of three locations AND the difference is due to your sampling effort, 
rather than to instrisic 
differences in local abundance, you can't sensibly compare their raw species 
richness counts, but 
you can if you rarefy the '150' and '200' samples down to 100 individuals. One 
might also want to 
do this before comparing the sites using a DIVERSITY index, assuming that the 
diversity index 
takes into account overall species richness (as they mostly all do).

So, rarefaction is a kind of 'pre-processing' step, rather than an alternative, 
to a diversity index.

Also, I can't think of a use for it in the context of equal sample sizes, 
unless you are simply 
interested the shapes of the rarefaction curves for the different sites. But 
those shapes are 
determined by the abundance distributions, so it would be more straightforward 
to compare the 
distributions directly.

Hope this helps,

Gareth Russell
NJIT/Rutgers

On Tue, 2 Jan 2007 13:55:51 -0500, bonnie clark <[EMAIL PROTECTED]> wrote:

>Dear Colleagues,
>I'm considering using rarefaction as a measure of species diversity, since
>it takes both species richness and species abundance into account.  There
>are several benefits of rarefaction over other indices like Shannon
>diversity.
>
>It is usually used when sample size is uneven.  Would it be inappropriate to
>use it when sample sizes are even (equal)?
>
>Thank you,
>Bonnie
>
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