Hello,

I am performing bottle incubation studies to examine the grazing of
copepods on algae and protists (microplankton). For each treatment I
have several replicate bottles, but I have been noticing that the
concentration of microplankton varies between "replicates" before I
even perform the incubation.

I have been exploring different methods for mixing the water before
dividing it into the bottles, and methods for putting the water into
the bottles. Literature for these types of studies gives little to no
information on the methods used to ensure good replication. If anyone
on the list has experience with this sort of problem I would
appreciate your ideas and advice.

Thanks very much,
Karen

-- 
Karen Kayfetz
Graduate Student
Romberg Tiburon Center for Environmental Studies
San Francisco State University
3150 Paradise Drive
Tiburon, CA 94920

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