Hi, When using remap, I prefer to use the '-noreverse' flag so that the translation of my DNA is located closer to my DNA strand. However, using this flag also remove the complementary strand of my DNA in the output which is less convinient when design primers. Is there a way in remap to display double-stranded DNA but turn off the restriction sites of the complementary strand?
If not, is there a program in EMBOSS which can retrieve the sequence from database, select start/end points and display both strands? I tried seqret but failed. Thank you in advance. Jean Mao _______________________________________________ EMBOSS mailing list [email protected] http://lists.open-bio.org/mailman/listinfo/emboss
