Hi Jean, > When using remap, I prefer to use the '-noreverse' flag so that the > translation of my DNA is located closer to my DNA strand. However, using > this flag also remove the complementary strand of my DNA in the output which > is less convinient when design primers. Is there a way in remap to display > double-stranded DNA but turn off the restriction sites of the complementary > strand?
I am looking at remap changes at the moment, I will see what I can do. > If not, is there a program in EMBOSS which can retrieve the sequence from > database, select start/end points and display both strands? I tried seqret > but failed. Showseq does that. It has a bug at present (I noticed it this week - fixed in the next release) that makes it show additional bases up to the end of the last line. regards, Peter _______________________________________________ EMBOSS mailing list [email protected] http://lists.open-bio.org/mailman/listinfo/emboss
