Hello Aengus, Restrict will report enzymes with the same recognition site if the source REBASE database lists them as having different cut sites. That appears to be the case with your reported output. So, you do seem to be using it correctly and the results also seem to be correct.
Alan > > I seem to be having trouble with restrict not picking up -limit or am I > not using it correctly? > > I shouldnt be getting both BssKI and ScrFI should I or indeed both BseBI > and EcoRII ??? > > ######################################## > # Program: restrict > # Rundate: Wed 5 Dec 2007 13:17:08 > # Commandline: restrict > # -sitelen 4 > # -enzymes all > # -limit > # -blunt > # -single > # [-sequence] rs9584819.ff > # -outfile > /home2/bioadmin/projects/MANOJ/RESTRICT_RESULTS/rs9584819.ff.restrict > # Report_format: table > # Report_file: > /home2/bioadmin/projects/MANOJ/RESTRICT_RESULTS/rs9584819.ff.restrict > ######################################## > > #======================================= > # > # Sequence: rs9584819 from: 1 to: 101 > # HitCount: 24 > # > # Minimum cuts per enzyme: 1 > # Maximum cuts per enzyme: 1 > # Minimum length of recognition site: 4 > # Blunt ends allowed > # Sticky ends allowed > # DNA is linear > # Ambiguities allowed > # > #======================================= > > Start End Enzyme_name Restriction_site 5prime 3prime 5primerev > 3primerev > 13 17 BssKI CCNGG 12 17 . > . > 13 17 BseBI CCWGG 14 15 . > . > 13 17 ScrFI CCNGG 14 15 . > . > 13 17 EcoRII CCWGG 12 17 . > . > > > > > Regards > Aengus > > > > -- > ----------------------------------------------------------------------- > Aengus Stewart > Head of Bioinformatics and BioStatistics > Bioinformatics and BioStatistics Tel: +44 (0)20 7269 3679 > Cancer Research UK, Lincoln's Inn Fields, Holborn, London, WC2A 3PX, UK > ----------------------------------------------------------------------- > > This electronic message contains information which may be privileged and > confidential. The information is intended to be for the use of the > individual(s) or entity named above. Be aware that any third party > disclosure, distribution, copying or use of this communication, without > prior permission, is strictly prohibited. > _______________________________________________ > EMBOSS mailing list > [email protected] > http://lists.open-bio.org/mailman/listinfo/emboss > _______________________________________________ EMBOSS mailing list [email protected] http://lists.open-bio.org/mailman/listinfo/emboss
