Yeah I know, not one of my brightest days............... Helps to look at cut position as well as motif *sigh*
Aengus Aengus Stewart wrote: > I seem to be having trouble with restrict not picking up -limit or am I not > using it correctly? > > I shouldnt be getting both BssKI and ScrFI should I or indeed both BseBI and > EcoRII ??? > > ######################################## > # Program: restrict > # Rundate: Wed 5 Dec 2007 13:17:08 > # Commandline: restrict > # -sitelen 4 > # -enzymes all > # -limit > # -blunt > # -single > # [-sequence] rs9584819.ff > # -outfile > /home2/bioadmin/projects/MANOJ/RESTRICT_RESULTS/rs9584819.ff.restrict > # Report_format: table > # Report_file: > /home2/bioadmin/projects/MANOJ/RESTRICT_RESULTS/rs9584819.ff.restrict > ######################################## > > #======================================= > # > # Sequence: rs9584819 from: 1 to: 101 > # HitCount: 24 > # > # Minimum cuts per enzyme: 1 > # Maximum cuts per enzyme: 1 > # Minimum length of recognition site: 4 > # Blunt ends allowed > # Sticky ends allowed > # DNA is linear > # Ambiguities allowed > # > #======================================= > > Start End Enzyme_name Restriction_site 5prime 3prime 5primerev > 3primerev > 13 17 BssKI CCNGG 12 17 . > . > 13 17 BseBI CCWGG 14 15 . > . > 13 17 ScrFI CCNGG 14 15 . > . > 13 17 EcoRII CCWGG 12 17 . > . > > > > > Regards > Aengus > > > -- ----------------------------------------------------------------------- Aengus Stewart Head of Bioinformatics and BioStatistics Bioinformatics and BioStatistics Tel: +44 (0)20 7269 3679 Cancer Research UK, Lincoln's Inn Fields, Holborn, London, WC2A 3PX, UK ----------------------------------------------------------------------- This electronic message contains information which may be privileged and confidential. The information is intended to be for the use of the individual(s) or entity named above. Be aware that any third party disclosure, distribution, copying or use of this communication, without prior permission, is strictly prohibited. _______________________________________________ EMBOSS mailing list [email protected] http://lists.open-bio.org/mailman/listinfo/emboss
