Dear EMBOSS experts

How good a shuffler is shuffleseq? I have always believed that it shuffles beyond all recognition, since tracer words inserted into a database are always gone after 1 round of shuffling. But are there any hard test data on its shuffling strength? For instance, for any, say, 100-mer, what fragments would reasonably be expected to remain after shuffling? Should I be surprised to see a 15-mer from the 100-mer still intact??

Sorry I can't express this more precisely. I thinks it's probably clear... I ask as there is a piece of software I am testing on real genomes and their shuffled derivatives, and I want to exclude the possibility that the control is compromised by an inadequacy of shuffling.

Cheers
Derek

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