Hi, [email protected] schrieb am 23/02/2010 00:21:38:
> I have a few questions on EMBOSS restriction analysis and will > appreciate any ideas or thoughts on these. > > 1. What Rebase file we need to download to get "restrict" working? I > tried but there are files with different formats. Go to the /pub/rebase dir on ftp.neb.com. Download the withrefm.xxx and proto.xxx files (xxx stands for the version number, just take the latest that's there) Run rebaseextract -infile withrefm.xxx -protofile proto.xxx This reformats the neb files for use with emboss. You should now see 4 files embossre.... in the REBASE directory > 2. Is there a maximum size limit of a nucleotide sequence that I can > use? Can I use the whole Human genome or at least a full chromosome > to digest with a particular restriction enzyme? I'm not sure about the whole genome but I have used it for individual chromosomes without problems. > 3. What program can give me the list of all possible fragments > generated as well? Since I have not seen the output of "restrict", > perhaps that is already doing that. You can run restrict with the option -fragments to get them. Hope this helps, David. _______________________________________________ EMBOSS mailing list [email protected] http://lists.open-bio.org/mailman/listinfo/emboss
