On 30/03/2010 12:46, Peter wrote:
Hi all,
I've got some "Sanger" capillary sequence files in ABI trace file
format, which I understand includes the probabilities of the 4 bases
along the sequencing run. I'd like to extract this as a FASTQ file
with meaningful quality scores based on the trace data (for use in
assembly).
This doesn't seem to work - the FASTQ quality score characters are all
double quotes (ASCI 34), meaning PHRED quality 1.
We have code to extract various fields from ABI trace files, but I'm not
familiar with the details fo the format, and documentation appears hard to
find.
Where do I look to find scores that we can use (and how do we convert those
to phred quality scores)?
regards,
Peter
_______________________________________________
EMBOSS mailing list
[email protected]
http://lists.open-bio.org/mailman/listinfo/emboss
