As seen in this discussion [https://www.biostars.org/p/102268/] primer3 (primer3_core) needs the SEQUENCE_TEMPLATE to be set in a config file. Apparently eprimer does not do this.
This discussion [https://www.mail-archive.com/[email protected]/msg01980.html] is referenced in the above thread to support the claim that primer32 should be used instead, but it actually states that the proper function to use for primer3-2.3.* is primer3. My versions: emboss-6.6.0 primer3-2.3.5 Could you help me out?
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