Extension of cell life-span and telomere length in animals cloned from senescent somatic cells.
Lanza RP, Cibelli JB, Blackwell C, Cristofalo VJ, Francis MK, Baerlocher GM, Mak J, Schertzer M, Chavez EA, Sawyer N, Lansdorp PM, West MD.
Advanced Cell Technology, One Innovation Drive, Worcester, MA 01605, USA. [EMAIL PROTECTED]
The potential of cloning depends in part on whether the procedure can reverse cellular aging and restore somatic cells to a phenotypically youthful state. Here, we report the birth of six healthy cloned calves derived from populations of senescent donor somatic cells. Nuclear transfer extended the replicative life-span of senescent cells (zero to four population doublings remaining) to greater than 90 population doublings. Early population doubling level complementary DNA-1 (EPC-1, an age-dependent gene) expression in cells from the cloned animals was 3.5- to 5-fold higher than that in cells from age-matched (5 to 10 months old) controls. Southern blot and flow cytometric analyses indicated that the telomeres were also extended beyond those of newborn (<2 weeks old) and age-matched control animals. The ability to regenerate animals and cells may have important implications for medicine and the study of mammalian aging.
PMID: 10784448 [PubMed - indexed for MEDLINE]
On Apr 28, 2005, at 10:30 AM, Vaj wrote:
Dr. Michael West's corporate web site for Advanced Cell Technologies
and their scientific papers are at:
http://www.advancedcell.com/Scientific-papers.htm
