I have heard of this before, but it was not as accurate. I guess they are working to make it more accurate? Either way, it's always been known to be in the saliva, but it dies so quickly that that is not how it is typically transmitted. It's more typically transmitted sexually like felv, through bad fighting like is involved in non neutered cats, and through birth to a positive mother. My positives have always groomed my negatives.
t
wendy <[EMAIL PROTECTED]> wrote:
wendy <[EMAIL PROTECTED]> wrote:
Did anyone read the post that Elizabeth sent on
testing saliva instead of blood for FeLV? It's below
in case you deleted it. I think it's very
interesting. Not only can they possibly test without
taking blood, but if FeLV is hanging out in the saliva
then it IS possible to pass it along via grooming (ie.
heavy petting...lol). What do you guys think about
this new research?
:)
Wendy
--- [EMAIL PROTECTED] wrote:
> An abstract from March 2006. Sure would make life
> easier if we could test
> without drawing blood....
>
>
> _J Clin Microbiol._ (_javascript_:AL_get(this,
> 'jour', 'J Clin
> Microbiol.');) 2006 Mar;44(3):916-22. _Related
> Articles,_
>
(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Display&dopt=pubmed_pubmed&from_uid=16517876)
>
> _Links_ (_javascript_:PopUpMenu2_Set(Menu16517876);)
>
>
>
(http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3051&uid=16517876&db=pubmed&url="">>
> Detection of feline leukemia virus RNA in saliva
> from naturally infected
> cats and correlation of PCR results with those of
> current diagnostic methods.
>
> _Gomes-Keller MA_
>
(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubmed_Abstract&term="Gomes-Keller+MA"[Author])
> , _Gonczi E_
>
(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubme
> d_Abstract&term="Gonczi+E"[Author]) , _Tandon R_
>
(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubmed_Abstract&term="Tandon+R"[
> Author]) , _Riondato F_
>
(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubmed_Abstract&term="Riondato+F"[Author])
> ,
> _Hofmann-Lehmann R_
>
(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubmed_Abstract&term="Hofmann-Lehmann+R"[Author])
> , _Meli ML_
>
(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubmed_Abstrac
> t&term="Meli+ML"[Author]) , _Lutz H_
>
(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Search&itool=pubmed_Abstract&term="Lutz+H"[Author])
> .
>
> Clinical Laboratory, Vetsuisse Faculty, University
> of Zurich,
> Winterthurerstrasse 260, 8057, Zurich, Switzerland.
> [EMAIL PROTECTED]
>
> A novel diagnostic test for feline leukemia virus
> (FeLV) RNA in saliva from
> naturally infected cats is described in this study.
> We evaluated different
> diagnostic tests and compared them with the widely
> used enzyme-linked
> immunosorbent assay (ELISA) for the detection of p27
> in the diagnosis of FeLV. Blood
> samples from 445 cats were tested for the presence
> of provirus by real-time
> PCR and plasma and saliva specimens from those cats
> were tested for the
> presence of viral RNA by real-time reverse
> transcription (RT)-PCR and for the
> presence of p27 by ELISA. In comparison to
> conventional ELISA, the diagnostic
> sensitivity and specificity of the detection of
> salivary FeLV RNA by real-time
> RT-PCR were found to be 98.1 and 99.2%,
> respectively. Detection of viral RNA in
> saliva had a positive predictive value of 94.6% and
> a negative predictive
> value of 99.7%. The kappa value was 0.96,
> demonstrating an almost perfect
> agreement between both tests. Furthermore, we
> confirmed previous results showing
> that a number of cats which tested negative for the
> presence of p27 in plasma
> were in fact positive for the presence of DNA
> provirus in blood specimens
> (5.4%). However, 96.4% of these latently infected
> cats did not shed viral RNA in
> saliva; therefore, we assume that these cats are of
> relatively low clinical
> importance at the time of testing. This study shows
> considerable diagnostic
> value of the detection of saliva FeLV RNA in
> naturally infected cats. This new
> diagnostic method has advantages over the
> conventional ELISA, such as less
> invasive sample collection and no requirement for
> trained personnel.
>
>
> _Entrez PubMed_
>
(http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16517876&query_hl=1&itool=pubmed_docsum)
>
>
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