No idea why. What is the difference in the headers? Can you post the 
terminal output of both your mri_vol2surf commands? Also, you can try 
using MRIread.m and MRIwrite.m (these use load_nifti and save_nifti 
under the hood but also do some things to the header).

doug


On 11/20/2013 08:46 AM, Leila Reddy wrote:
> Hi,
>
> I am trying to save a nifti file using save_nifti. I'm running into a 
> strange problem that I describe below.
>
> I have a nifti file (subj_mask.nii) created by a colleague using 
> Nibabel (http://nipy.sourceforge.net/nibabel/). This volume is in the 
> MNI305 space.
>
> Step1: In a first step I wanted to convert this volume to the 
> fsaverage surface format. To do that I used :
>
>  mri_vol2surf --src subj_mask.nii --hemi rh --out_type mgh --float2int 
> round --o blah9-rh.mgh --projfrac 0.5 --trgsubject fsaverage 
> --regheader fsaverage.
>
> I load it in tksurfer and when I do view-> configure->overlay, I see a 
> range of values (as expected) and am able to set the min and max 
> thresholds as I want.
>
> Step 2: In a second step I loaded this nifti file into matlab using 
> load_nifti and had to alter the volume a little bit as below:
> hdr=load_nifti('subj_mask.nii');
> vol=hdr.vol;
> vol(find(vol<4))=0;
> hdr.vol=vol;
> save_nifti(hdr,'new_mask.nii').
>
> I convert the volume to fsaverage surface space as before but when I 
> view it in tksurfer I am not able to set a min and max threshold with 
> configure->overlay. Instead, there is no distribution of values 
> plotted in the configure overlay window and I get the following error 
> message: % ERROR updating histo: impossible limits (min 0.55556 >= max 
> 0.55556) for axis "x"
>
> I finally managed to solve this problem by loading the header 
> information from a FS-FAST created nifti:
>
> hdr_fsfast = load_nifti('bold/analysis.mni/mask.nii.gz');
> hdr_fsfast.vol=vol;
> save_nifti(hdr_fsfast,'new_mask.nii').
>
> Now I am able to see the distribution of values in the configure 
> overlay window and am able to set the thresholds.
>
> The headers for FS-FAST and Nibabel generated niftis are indeed 
> slightly different but why do I not have the problem in step 1 when I 
> use the Nibabel file, but only in step 2 after I use save_nifti with 
> the Nibabel header ?
>
> I use the load_nifti and save_nifti commands very often (and haven't 
> had problems before because I was only using FS-FAST generated files) 
> so I would really appreciate it if someone could point out what is 
> going on so that I understand what to do in future.
>
> Thanks,
> Leila
>
>
>
>
> _______________________________________________
> Freesurfer mailing list
> [email protected]
> https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer

-- 
Douglas N. Greve, Ph.D.
MGH-NMR Center
[email protected]
Phone Number: 617-724-2358
Fax: 617-726-7422

Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
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