Hi Doug, 

thanks for your reply. It made things a lot clearer. I totally understand that 
you're probably receiving more than one cry for help per day.

What would you say are the conventions for picking a threshold for analyses on 
structural data? 

Cheers, Clara

----- Ursprüngliche Mail -----
Von: "Douglas N Greve" <[email protected]>
An: [email protected]
Gesendet: Mittwoch, 13. April 2016 00:15:41
Betreff: Re: [Freesurfer] REPOST: Monte Carlo correction in QDEC

sorry, I could have sworn that I answered this one

On 04/12/2016 08:57 AM, Clara Kühn wrote:
> Dear FreeSurfer experts,
>
> for the analysis in QDEC I created my own Monte Carlo correction.
> My questions relate to the threshold option.
>
> 1. Would I use neg if I have mostly blue clusters in the QDEC display and pos 
> if I have mostly red clusters?
No, you would use neg when you have an apriori assumption that your 
effect is going to be negative. Once you look at the results, it is no 
long apriori
>
> 2. When do I use abs?
If you do not have an apriori assumption about the sign of the effect
>
> 3. I compared the neg option at different thresholds (1.3, 2.0 and 2.3) and I 
> get different clusters:
> 1.3 (=.05)
> # ClusterNo  Max   VtxMax   Size(mm^2)  MNIX   MNIY   MNIZ    CWP    CWPLow   
>  CWPHi   NVtxs   Annot
>     1       -2.669   76591   1148.00     -6.7   14.5   62.7  0.00880  0.00760 
>  0.01000  1600  superiorfrontal
>     2       -2.443   91912   2128.78    -10.3   55.3  -23.5  0.00010  0.00000 
>  0.00020  2971  medialorbitofrontal
>
> 2.0 (=.01)
> # ClusterNo  Max   VtxMax   Size(mm^2)  MNIX   MNIY   MNIZ    CWP    CWPLow   
>  CWPHi   NVtxs   Annot
>     1       -3.289   92754    420.03    -36.5   57.0  -21.0  0.02140  0.01960 
>  0.02330   571  parsorbitalis
>     2       -2.669   76591    428.11     -6.7   14.5   62.7  0.01940  0.01760 
>  0.02120   604  superiorfrontal
>
> 2.3 (=.005)
> # ClusterNo  Max   VtxMax   Size(mm^2)  MNIX   MNIY   MNIZ    CWP    CWPLow   
>  CWPHi   NVtxs   Annot
>     1       -3.289   92754    314.80    -36.5   57.0  -21.0  0.01900  0.01730 
>  0.02080   410  parsorbitalis
>
> Technically, if parsorbitalis is significant at .01 and .005, shouldn't it 
> also be significant at .05? I've compared the clusters in freeview and the 
> superiorfrontal is the same cluster in both 1.3 and 2.0. The parsorbitalis 
> and the medialorbitofrontal clusters are completely different, not even 
> closely overlapping.
You are confusing the two types of thresholds. The voxel-wise threshold 
that you are changing defines what is and is not a cluster. As you 
change it clusters will change size. As you make it more liberal, you 
make it more likely that you see a cluster of a certain size by chance 
(ie, the p-value for the cluster gets worse). So as you make the 
threshold more liberal, there are two competing effects: (1) the cluster 
gets bigger, and (2) the p-value of a cluster of a given fixed size gets 
worse. If the cluster size does not increase enough to overcome the 
second effect, then the cluster p-value will get worse. It is just very 
complicated.

>
> Do you have any idea why that is and what it does differently?
> Thank you!
> Cheers, Clara
> _______________________________________________
> Freesurfer mailing list
> [email protected]
> https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer
>
>

-- 
Douglas N. Greve, Ph.D.
MGH-NMR Center
[email protected]
Phone Number: 617-724-2358
Fax: 617-726-7422

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