Hi Michelle

sorry you are having so much trouble! Can you give us some details of you acquisition? We don't usually see this kind of persistent issues
cheers
Bruce

On Fri, 22 Dec 2017, Michelle VanTieghem wrote:


Hi Douglas, 

I have reported many problems with the skull stripping and resulting issues in 
freesurfer. to
summarize:

1. For our structural scans, we have significant pial matter/dura matter 
remaining for about 50% of
subjects or more (of a dataset of 350 scans). I  have tested using freesurfer's 
gcut and other
options, as well as AFNI skull strip, FSL with many options, and even ANTS. 

2. We gave up on trying to use surfaces with this dataset, due to the sheer 
amount of manual
intervention that would be needed to edit the brains

3. The aseg almost always labels the remaining skull as gray matter, which then 
influences the icv
and wbv measurements. W

4. Recently we have decided to try the longitudinal pipeline. Another problem 
appears: if a
cross-sectional scan does not have any pial matter (as in, it is one of the few 
that as successfully
skull stripped -  we call these the "clean" scans) but a template is made from 
multiple scans from
this subject. If one of those other tp scans from this subject has any pial 
mater remaining, then
the (a) the template will include all of the pial matter and (b) the 
longitudinal version of the
"clean" scan now has extra pial matter that gets erroneously labeled as gray 
matter, or sometimes
white matter. Attached is a screenshot example of this problem.

I have been trying to solve this for over a year, and I also sent data to 
freesurfer over the ftp on
December 1st using the directions on the freesurfer wiki, if you want to take a 
look at a single
representative scan with this skull strip issue.

Thanks!

Michelle 


----

Douglas N Greve Tue, 19 Dec 2017 15:25:14 -0800

Hi Michelle, sorry about not getting to this before. It looks like Bruce was helping you out, but he's just left and won't be back until the new year. To answer your first question, I don't think that v6 performs much differently on skull stripping than v5.3. What is the nature of the skull stripping problem? Can you send screen shots?

doug


On Tue, Dec 19, 2017 at 3:47 PM, Michelle VanTieghem 
<[email protected]> wrote:
      Hi, 
I am following up again --  on my prior posts regarding skull strip issues and 
whether
using freesurfer 6.0 would improve over freesurfer 5.3 . 
I sent the data over the ftp on  Dec 1 and would greatly appreciate it if you 
could take a
look to see if there is a way to improve the skull strip since we have this 
issue with 100+
scans and my attempts to use gcut, and other skullstrip programs, has not 
worked.

Thank you,
Michelle 

On Fri, Dec 8, 2017 at 12:31 PM, Michelle VanTieghem 
<[email protected]> wrote:
      Hi, 
I am just following up on my prior posts regarding skull strip issues and 
whether using
freesurfer 6.0 would improve over freesurfer 5.3 . 
I sent the data over the ftp and would greatly appreciate it if you could take 
a look to
see if there is a way to improve the skull strip since we have this issue with 
100+
scans and my attempts to use gcut, and other skullstrip programs, has not 
worked.

Thank you,
Michelle 

On Fri, Dec 1, 2017 at 5:08 PM, Michelle VanTieghem 
<[email protected]>
wrote:
      Ok that worked. I sent the data for a subject with the skull stripping 
issue
      that is common in most of our dataset.
Thanks,
Michelle

On Fri, Dec 1, 2017 at 4:56 PM, Bruce Fischl <[email protected]> wrote:
      try cd'ing to incoming not outgoing (the names are from our
      perspective I think :)

      On Fri, 1 Dec 2017, Michelle VanTieghem wrote:


            yn-128-59-230-36:structural michellevantieghem2$ ftp
            surfer.nmr.mgh.harvard.edu

            Connected to surfer.nmr.mgh.harvard.edu.

            220-This is the Martinos Center Anonymous FTP service. Use
            of this site             

            220-shows implicit consent to our Acceptable Use Policy
            outlined at               

            
220-http://www.nmr.mgh.harvard.edu/martinos/userInfo/computer/nmr-aup.php 
                     

            220-

            220-OPERATIONAL NOTE:  If, when doing a GET or PUT, you
            encounter either 

            220-

            220-229 Entering Extended Passive Mode

            220-

            220-                   and nothing happens OR 

            220-

            220-229 Entering Extended Passive Mode

            220-500 Bad EPRT protocol. 

            220-

            220-                  try issuing the EPSV command prior
            to GET or PUT.

            220-

            220 

            Name (surfer.nmr.mgh.harvard.edu:michellevantieghem2):
            anonymous

            331 Please specify the password.

            Password: 

            230 Login successful.

            Remote system type is UNIX.

            Using binary mode to transfer files.

            ftp> cd transfer/outgoing

            250 Directory successfully changed.

            ftp> put SB152_freesurfer.tar.gz 

            local: SB152_freesurfer.tar.gz remote:
            SB152_freesurfer.tar.gz

            229 Entering Extended Passive Mode (|||37860|).

            553 Could not create file.

            ftp> ls

            229 Entering Extended Passive Mode (|||37896|).

            150 Here comes the directory listing.

            -rw-r--r--    1 0        0            3812 Dec 01 06:10
            README.1st

            drwxrwsr-t    4 0        1046         4096 Nov 21  2014
            flat

            drwxrwsr-t   13 0        160          4096 Aug 19  2013
            fsdev

            drwxrwsr-t   14 0        1046         4096 Dec 05  2011
            recon

            drwxrwsr-t    3 0        18           4096 Jan 24  2005
            sysadm

            226 Directory send OK.

            ftp> ls

            229 Entering Extended Passive Mode (|||37892|).

            150 Here comes the directory listing.

            -rw-r--r--    1 0        0            3812 Dec 01 06:10
            README.1st

            drwxrwsr-t    4 0        1046         4096 Nov 21  2014
            flat

            drwxrwsr-t   13 0        160          4096 Aug 19  2013
            fsdev

            drwxrwsr-t   14 0        1046         4096 Dec 05  2011
            recon

            drwxrwsr-t    3 0        18           4096 Jan 24  2005
            sysadm

            226 Directory send OK.

            ftp> put SB152_freesurfer.tar.gz

            local: SB152_freesurfer.tar.gz remote:
            SB152_freesurfer.tar.gz

            229 Entering Extended Passive Mode (|||37898|).

            553 Could not create file.

            ftp> 


            On Fri, Dec 1, 2017 at 4:50 PM, Bruce Fischl
            <[email protected]> wrote:
                  can you paste the full session text into the email
            (not as a screen shot)?

                  On Fri, 1 Dec 2017, Michelle VanTieghem wrote:

                        Hi, I am unsure whether this worked: see
            attached screenshot.

                        Thanks!
                        M

                        On Fri, Dec 1, 2017 at 4:26 PM, Bruce Fischl
            <[email protected]> wrote:
                              yes, it should. You should just be able
            to do a "put"
                              On Fri, 1 Dec 2017, Michelle VanTieghem
            wrote:

                                    Hi, 
                                    I am confused by the instructions
            for the ftp. Once I am logged into the ftp and have
                        navigated to
                                    the transfer/incoming
                                    directory, how am I supposed to
            find / access the file that I would ike to send
                        through? Does
                                    terminal still recognize my
                                    working directory before I logged
            into the ftp?

                                    Thanks!
                                    Michelle 

                                    On Sat, Nov 18, 2017 at 2:54 PM,
            Bruce Fischl <[email protected]> wrote:
                                          Hi Michelle

                                          probably best to tar and
            gzip the entire subject directory and ftp it to us:

                                         
            https://surfer.nmr.mgh.harvard.edu/fswiki/FtpFileExchange

                                          cheers
                                          Bruce

                                          On Sat, 18 Nov 2017,
            Michelle VanTieghem wrote:

                                                Hi, 
                                                Yes I can send you the
            data - should I just attach the mprage file in the
                        email, or is
                                    there
                                                another
                                                way to share the data
            with freesurfer? Also, should I send one scan or
                        multiple
                                    scans? 

                                                Thanks,
                                                Michelle

                                                On Sat, Nov 18, 2017
            at 10:37 AM, Bruce Fischl
                        <[email protected]> wrote:
                                                      Hi Michelle

                                                      are you doing
            anything out of the ordinary? Do you want to send us a
                        dataset and
                                    we will
                                                      take a look? I
            don't think 6.0 is very different from 5.3 in regard
                        to skull
                                    stripping,
                                                      although it is
            probably a bit better

                                                      Bruce

                                                      On Fri, 17 Nov
            2017, Michelle VanTieghem wrote:

                                                            Hello, 
                                                            I have
            posted several times about problems with
                        skull-stripping with our
                                                            dataset.
            We have tried gcut
                                                            and
            multiple other options in other packages (AFNI, FSL, ANTS)
                        to remove
                                                            skull from
            structural
                                                            images in
            our dataset with little success.

                                                            Recently,
            a colleague suggested this may be due to versions:
                        that 6.0
                                    deals
                                                            with skull
            stripping
                                                            much
            better than 5.3. 

                                                             I wanted
            to ask if indeed this could be the case before I go
                        install 6.0
                                                            and re-run
            everything in
                                                            my study.

                                                            Thank
            you, 
                                                            Michelle 

                                                            --
                                                            Michelle
            VanTieghem
                                                            PhD
            student in Psychology
                                                           
            Developmental Affective Neuroscience Lab
                                                            Columbia
            University 
                                                           
            [email protected]




                                                The information in
            this e-mail is intended only for the person to whom it
                        is
                                                addressed. If you
            believe this e-mail was sent to you in error and the
                        e-mail
                                                contains patient
            information, please contact the Partners Compliance
                        HelpLine at
                                               
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                        properly
                                                dispose of the e-mail.




                                                --
                                                Michelle VanTieghem
                                                PhD student in
            Psychology
                                                Developmental
            Affective Neuroscience Lab
                                                Columbia University 
                                                [email protected]




                                    --
                                    Michelle VanTieghem
                                    PhD student in Psychology
                                    Developmental Affective
            Neuroscience Lab
                                    Columbia University 
                                    [email protected]




                        --
                        Michelle VanTieghem
                        PhD student in Psychology
                        Developmental Affective Neuroscience Lab
                        Columbia University 
                        [email protected]




            --
            Michelle VanTieghem
            PhD student in Psychology
            Developmental Affective Neuroscience Lab
            Columbia University 
            [email protected]




--
Michelle VanTieghem
PhD student in Psychology
Developmental Affective Neuroscience Lab
Columbia University 
[email protected]




--
Michelle VanTieghem
PhD student in Psychology
Developmental Affective Neuroscience Lab
Columbia University 
[email protected]




--
Michelle VanTieghem
PhD student in Psychology
Developmental Affective Neuroscience Lab
Columbia University 
[email protected]




--
Michelle VanTieghem
PhD student in Psychology
Developmental Affective Neuroscience Lab
Columbia University 
[email protected]

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