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Dear Douglas,Thank you for the reply,The MDROI.nii is a set of ROIs, which I 
want to transform in separate labels. So how can I extract the ROIs?? I already 
tried:
mri_extract_label MDROI.nii 1 label1.mgz
but it extracts the labels according to the inherent anatomical ROIs from 
freesurfer 
(https://surfer.nmr.mgh.harvard.edu/fswiki/FsTutorial/AnatomicalROI/FreeSurferColorLUT),
 and not the ones the volume is showing.
I also tried:
mri_annotation2label --subject MNI_1mm/ --annotation MDROI.nii --outdir 
MNI_1mm/trial_MD_2_labels/
but it needs a corresponding .annot file for the --annotation argument   On 
Friday, August 7, 2020, 05:11:40 PM GMT+3, Douglas N. Greve 
<dgr...@mgh.harvard.edu> wrote:  
 
  The hemi just refers to either lh or rh to load the proper surface. The 
command have no idea what you are transferring to the surface.
 
 Before the mri_vol2label stage, try loading surf.MDROI  onto the surface as an 
overlay and see if the ROIs are showing up in the right place
 
 
 On 8/6/2020 11:41 AM, Eleni Nikalexi wrote:
  
 
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  Dear Freesurfer developers, We have a volume that assigns unique numerical 
labels to each of the ROIs it contains, in NIFTI format. I want to register it 
to MNI152 (1mm) space, so that I can map it on my subjects and use 
mris_anatomical_stats. I have tried the following command:  
  1. tkregisterfv --mov MDROI.nii --s MNI_1mm --regheader --reg 
registrationMD/MDROI.lta
  
  to get :  "LTA input is not RAS to RAS...converting...".
   Then I run: 
          2. mri_vol2surf --src MDROI.nii --src_type nii --out 
registrationMD/surf.MDROI.mgh --reg             registrationMD/MDROI.lta --hemi 
lh --surf white --projdist-max 0 1 0.1          3. mri_vol2surf --src MDROI.nii 
--src_type nii --out registrationMD/surf.MDROI_rh.mgh --reg             
registrationMD/MDROI.lta --hemi rh --surf white --projdist-max 0 1 0.1  The 2 
commands above demand the --hemi argument, but I wasn't sure what to put, as 
the ROIs are mixed and are in both hemispheres. Thus I run the command once for 
the left and once for the right hemisphere, creating different .mgh files. 
Last, I continued with:
  
          4. mri_vol2label --i registrationMD/surf.MDROI.mgh --id 1 --l 
lab.MDROI_lh.label --surf MNI_1mm l 
  which failed, as I get "ERROR: found no voxels matching id 1" 
  Could you please tell me, if the commands make sense, and if I should change 
something?
 
  Thank you so much for your help! 
  Best regards, Eleni   
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