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Dear freesurfer developers, 

I am working on preprocessing resting state fMRI and used the following command.

$ preproc-sess -s sess01 -fsd rest -stc siemens -surface fsaverage lhrh 
-surf-fwhm 5 -mni305-2mm -vol-fwhm 0 -per-session 

When I tried to visualize f.nii.gz with registration file applied and overlaid 
with aparc+aseg.mgz from structural analysis, they do not overlap. It seems 
like the orientation is not the same, and the f.nii.gz with registration seems 
to be 90 degree rotated from apjarc+ aseg. mgz.  (I wish I could attach a 
screenshot, but last time I tried, it says my email exceeded the capacity and 
sent to the moderator) 

I also tried bbregister. I was suggested not to use —init-rr, so I tried all 
the other registration methods, but same problem occurs. 

$bbregister --mov sess01/rest/001/template.nii.gz --t2 --s subject --lta 
register.dof6.lta 

—init-rr does a good job when I checked visually by applying registration on 
f.nii.gz and checked if it overlaps with structural scan. But, I thought the 
registration file goes both ways, but when I checked structural scan (orig.mgz 
or aparc+aseg.mgz) with registration file and overlapped with functional scan 
(f.nii.gz), it does not overlap. 

Is there a specific way that I have to apply a registration file to visualize 
the transformation in the opposite direction? 
If preprocess registration does not work, is there any other method that I can 
try to make them aligned? 

Thank you  for your time and help ! 

Best, 
Wenzhen 


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