Ahhh - I get it now. Galaxy only adds itself to the PYTHONPATH if it
can infer it is running a Python script (i.e. the command block starts
with python or a Python interpreter is used).

If you are building a tool for the tool shed - it would be best to
setup a tool dependency on the galaxy_sequence_utils package - this
would force every execution of the tool to have these utilities on
your PYTHONPATH. But since you are hard-encoding paths - it looks like
you are tailoring your Galaxy instance to your local environment. In
this case an easy hack is to just setup that dependency globally for
the system:

sudo easy_install
http://depot.galaxyproject.org/package/source/galaxy_sequence_utils/galaxy_sequence_utils-1.0.0.tgz

If you are setting up Galaxy env.sh files this can be installed into a
virtualenv instead - which is a better practice - let me know if you
are interested and I can send details.

-John




On Mon, Jul 7, 2014 at 4:02 AM, julie dubois <dubju...@gmail.com> wrote:
> Hi John,
>
> As I specified it in the "edit" of my first message, I have this error
> only if I run groomer with a command line in another xml file
> surrounding by other command. When I run groomer alone with the
> original xml, it works fine, so no problem with the PYTHONPATH.
>  But when I use this :
> <command>
>                 #if $fastq_format == "illumina"
>         blabla_No_python_command
>                 #else
>         python /data/galaxy-dist/tools/fastq/fastq_groomer.py
> 'tmp.fastqcssanger' 'cssanger' '$output' 'illumina' 'ascii'
> 'dont_summarize_input'
>                 #end if
> </command>
>
> It doesn't work.
>
> I've checked the files contained in job_working_directory. A shell
> script is generated for my job and it begin with these lines :
>
> #!/bin/sh
>
> GALAXY_SLOTS="1"; export GALAXY_SLOTS;
> export GALAXY_SLOTS
> GALAXY_LIB="None"
> if [ "$GALAXY_LIB" != "None" ]; then
>     if [ -n "$PYTHONPATH" ]; then
>         PYTHONPATH="$GALAXY_LIB:$PYTHONPATH"
>     else
>         PYTHONPATH="$GALAXY_LIB"
>     fi
>     export PYTHONPATH
> fi
>
>
>
> So, with the run of groomer alone the line GALAXY_LIB is equal to
> "/data/galaxy/galaxy-dist/lib", but here with the run of groomer with
> a command line with another job the GALAXY_LIB is set to "None"!!!
> I don't understand why.
>
> For the moment, to surround the problem, I've explicitly specified the
> GALAXY_LIB path int the xml command before the command line wich run
> python fastq_groomer.py and it works but I'm aware that is not
> beautiful.
>
> Finally I'm using a local jobrunner.
>
> Thanks for your help
> Julie
>
> P.S : we use such command because we build a workflow where the
> quality format of the fastq must be invisible for the users.
>
> 2014-07-03 19:48 GMT+02:00 John Chilton <jmchil...@gmail.com>:
>> Hello Julie,
>>
>>   This is odd - sorry.
>>
>>   I might be worth uninstalling and re-installing this in the tool
>> shed admin options - in particular ensure you install tool
>> dependencies and that this installation process succeeds.
>>
>>   Otherwise -  it might be worth trying to put some debug statements
>> in the fastq groomer tool to try to track down the problem. Here
>> (https://gist.github.com/jmchilton/2d61f7ab81d99a0f02fc) is a diff to
>> have that tool write out the run-time value  PYTHONPATH to Galaxy's
>> home directory - it might be worth running a job, finding the output,
>> setting PYTHONPATH to that value on the compute node and attempting to
>> just import it directly.
>>
>> cd $HOME; python -c "import galaxy.sequences.fastq"
>>
>>   Out of curiosity - what job runner are you using (local, DRMAA, etc...)?
>>
>> -John
>>
>> On Tue, Jul 1, 2014 at 4:50 AM, julie dubois <dubju...@gmail.com> wrote:
>>> Hi,
>>> I've this error message when I run groomer.
>>>
>>> My local instance of galaxy runs on a python virtualenv (python 2.7)
>>> and I have make the latest update with : hg pull -u.
>>>
>>> I've verified and this library is present in my
>>> galaxy-dist/lib/galaxy_utils/sequence directory.
>>>
>>> I've found no other idea than the update on this forum to run groomer
>>> correctly, any other idea ?
>>>
>>> Thanks.
>>> Julie
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