Hello Tao,

It seems there has been a misunderstanding. Originally in other communications, your data was explained to be "4 samples with 2.4 GB of seq data per sample". If your 4 samples are actually a set of files with a total size of 350GB each (350GB x 4), then combined this would exceed the maximum quota of 50G per file/200G per history at the public Galaxy instance at http://usegalaxy.org.

The option for this much data is to run either a local or cloud install. Help can be found at:
http://getgalaxy.org
http://galaxyproject.org/Admin/Cloud

Questions related to local or cloud installations can be sent to the galaxy-...@bx.psu.edu mailing list. Other users on this list may also have advice to offer about working with very large datasets.

Best wishes for your project,

Jen
Galaxy team


On 8/9/11 2:42 PM, Peng, Tao wrote:
Hi I am NEW to RNA-seq data and galaxy programs. When I try to up-load
the RNA-seq data using FileZilla using my desktop PC, each file
(about350 MB) takes about 1 hour to up-load.

Realizing I have 4 samples, each sample has 9 fasq.gz files, each file
is about 350 GB, I really need some advice how to make this up-load work.

Thanks,

Tao

tp...@fhcrc.org



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___________________________________________________________
The Galaxy User list should be used for the discussion of
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at usegalaxy.org.  Please keep all replies on the list by
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