Hi Daniel for 2) you may use the tools under NGS QC and manipulation FASTQ to FASTA<http://main.g2.bx.psu.edu/tool_runner?tool_id=cshl_fastq_to_fasta>converter
followed by Collapse<http://main.g2.bx.psu.edu/tool_runner?tool_id=cshl_fastx_collapser>sequences On 19 September 2011 09:54, Kevin Lam <ke...@aitbiotech.com> wrote: > For 1) you may refer to Simulated Dataset of Solexa - > SEQanswers<http://seqanswers.com/forums/showthread.php?t=806> > > > Has anyone replied you for 2) ? > > > > On 18 September 2011 21:12, Daniel Sher <ds...@sci.haifa.ac.il> wrote: > >> Hello, >> >> I have two questions - I apologize if they are trivial.. >> >> 1) I want to simulate the amount of Illumina sequencing needed to >> sequence and assemble a known genome. Is there a way to randomly pick >> sequences of a specific length from a genome (either one available online or >> one I upload)? Something like "pick 100bp randomly (either strand), move >> 400-500bp forward and pick another 100bp?" >> >> 2) Is there a way to remove redundant sequences from a FASTA file without >> losing the original sequence names (as happens with "collapse")? >> >> Thanks >> >> Daniel >> >> >> -- >> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ >> Daniel Sher, PhD >> Department of Marine Biology >> Leon H. Charney School of Marine Sciences >> University of Haifa, Mt. Carmel 31905, Haifa, Israel >> >> Office +972-4-8240731 >> Lab +972-4-8288961 >> email: ds...@sci.haifa.ac.il >> >> >> ___________________________________________________________ >> The Galaxy User list should be used for the discussion of >> Galaxy analysis and other features on the public server >> at usegalaxy.org. Please keep all replies on the list by >> using "reply all" in your mail client. For discussion of >> local Galaxy instances and the Galaxy source code, please >> use the Galaxy Development list: >> >> http://lists.bx.psu.edu/listinfo/galaxy-dev >> >> To manage your subscriptions to this and other Galaxy lists, >> please use the interface at: >> >> http://lists.bx.psu.edu/ >> > >
___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
