Hi there, I apologize if this is covered in documentation or help threads. searched and it seemed it was not. I have several illumina rna-seq data sets that "should" be directional. It seems the directionality is very good, based on the visualization. First question is; in the visualization window, are the reads color coded by direction, i.e. are orange one direction and blue the other? If so, it looks like my data is >95% directional. Similar question, is there a way to quantify directionality of the data set?
BTW, the data is RNA-seq data from rhesus macaques and i'm using the rhesus macaque genome build from UCSC. thanks very much, Nick Maness Tulane University
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