I am using Galaxy main site to analyse MiSeq data of pooled samples.
Essentially the run produces 3 fastq files consisting of R1, R2 read files and
a separate index file. They are in the format below.
R1: @M00132:6:000000000-A0JG4:1:1:18014:1842 1:N:0:0
R2: @M00132:6:000000000-A0JG4:1:1:18014:1842 2:N:0:0
Index: @M00132:6:000000000-A0JG4:1:1:18014:1842 1:N:0:0
I would like to use Galaxy to demultiplex the samples and then analyse them
individually. I have found barcode Splitter (version 1.0.0) on Galaxy however
this tool requires the index to be found at the beginning of the sequence.
Therefore I am attempting to add the index sequence onto the end of the
sequence read data. FASTQ joiner (version 1.0.0) joins fastq files, however the
fastqs to be joint must be distinguished by a /1 or /2 at end of sequence
identifiers. Does anyone have any advice or experience of demultiplexing data
in this format?
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