Hi galaxy,
I'm trying to know the coding potential of my RNA-seq transcripts by phylogeny 
analysis. So I want to generate a FASTA file that includes ORF of my sequence 
and 29 other mammalian species. I used"Extract MAF blocks" and "Stitch MAF 
blocks" using my RNA-seq data(gtf file from cuffcompare) as intervals. But the 
final FASTA file doesn't include my sequence. How can I add my sequence to the 
final FASTA so that it includes both the 29 mammalian genome and my sequence?
Thanks in advance.Lyni.                                           
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:


To manage your subscriptions to this and other Galaxy lists,
please use the interface at:


Reply via email to