Hi Aaron,

If you did this on our public main server, you can use the share options (gear 
icon in history list --> Share or publish), this will let us investigate a bit 
deeper into the exact problem/situation. 

If you were using a local instance (or the public server), then emailing them 
directly to me will work just fine.


Thanks for using Galaxy,

Dan


On Feb 8, 2013, at 3:10 PM, Aaron Quinlan wrote:

> Hi Dan,
> 
> Thanks for the follow up.  Yes, I was also able to get it to work when I 
> created a test case using the example I originally sent. Yet when I run the 
> entire files, I get zero intersections.  Join, in contrast, works fine.  I'd 
> be happy to share the files.  Would it be best to send them directly to you 
> by email?  The are small.
> 
> Thanks much for the help,
> 
> - Aaron
> quinlanlab.org
> 
> 
> 
> 
> 
> On Feb 8, 2013, at 2:56 PM, Daniel Blankenberg <d...@bx.psu.edu> wrote:
> 
>> Hi Aaron,
>> 
>> I just tested this small example and it reported one region as the result of 
>> the intersect: 
>> https://main.g2.bx.psu.edu/u/dan/h/aaron-quinlan-intersect-test-02-08-2013
>> 
>> Do you have a history available that you can share (privately if you desire) 
>> where you see the issue, and we'll take a look.
>> 
>> 
>> Thanks for using Galaxy,
>> 
>> Dan
>> 
>> 
>> On Feb 8, 2013, at 10:23 AM, Aaron Quinlan wrote:
>> 
>>> Dear list,
>>> 
>>> I have a student that found an unexplained discrepancy between the results 
>>> produced by the "Operate on Genomic Intervals" (OGI) intersect operation 
>>> versus the OGI join operation.  In particular, we know for certain that 
>>> there are exactly 1105 intersection of at least 1bp between the two files 
>>> we are testing, as we have confirmed this with our own bedtools and the 
>>> ucsc table browser.  An example intersection (intersecting positions: 
>>> 10012008 - 10012013):
>>> 
>>> file 1:
>>> chr1        10012008        10012021        5.6186
>>> 
>>> file 2:
>>> chr1        10011813        10012013        5_Strong_Enhancer       0       
>>> +       10011813        10012013        250,202,0
>>> 
>>> However, OGI intersect find 0 intersections between the files (settings: 
>>> return overlapping intervals, >= 1bp).  In an effort to make sure we didn't 
>>> goof up on file formats (BED) or genome builds (hg19), we tested the exact 
>>> same two files with the OGI join operation and found 1105 intersections as 
>>> expected.
>>> 
>>> I also tested the files with the bx-python bed_intersect.py and 
>>> bed_intersect_basewise.py scripts and get the expected results.
>>> 
>>> Does anyone have a suggestion for how to resolve this?
>>> 
>>> Thanks for your help and for providing such a fantastic resource to the 
>>> genomics community.
>>> 
>>> Best,
>>> - Aaron
>>> quinlanlab.org
>>> 
>>> 
>>> 
>>> 
>>> 
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> 

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