Hi all, Please help with the quality score type for the downloaded Solid datasets. I downloaded RNA-seq datasets, which were generated by AB Solid system, as base space and at FastQ format from SRA of NCBI. I uploaded the datasets onto the online sever Galaxy and change the datatype directly into "fastqsanger" and then test the quality by running FastQC. The output "per base quality" of solid dataset (please take look at the attached figure "per_base_quality-Solid") is quite different from the output "per base quality" of Illumina dataset (please compare with the attached figure "per base quality-Illumina"). The top score for Solid dataset is about 31, however the top score for Illumina dataset is 38. What is the quality score type for the downloaded Solid datasets when downloaded as base space and at FastQ format from SRA of NCBI? Please help me solve this problem.
Thanks. Best regards. Jianguang Du
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