Hi all, Please help with the quality score type for the downloaded Solid datasets. I downloaded RNA-seq datasets, which were generated by AB Solid system, as base space and at FastQ format from SRA of NCBI. I uploaded the datasets onto the online sever Galaxy and change the datatype directly into "fastqsanger" and then test the quality by running FastQC. The output "per base quality" of solid dataset (please take look at the attached figure "per_base_quality-Solid") is quite different from the output "per base quality" of Illumina dataset (please compare with the attached figure "per base quality-Illumina"). The top score for Solid dataset is about 31, however the top score for Illumina dataset is 38. What is the quality score type for the downloaded Solid datasets when downloaded as base space and at FastQ format from SRA of NCBI? Please help me solve this problem.
Thanks. Best regards. Jianguang Du
<<attachment: per_base_quality-Illumina.png>>
<<attachment: per_base_quality-Solid.png>>
___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
