Dear all,
My problem seems like something that should have a very simple solution from my 
end and due to my lack of knowledge in bioinformatics, I am probably messing up 
with the workflows. The experiment I run is one where we used Miseq to sequence 
amplicons of a multiplex PCR. We introduced an inhouse barcodeto our PCR 
products via an adaptor. 
 
Miseq data was demultiplexed for the Illumina barcodes using Miseq reporter on 
intrument software by our service provider and I am trying to run the rest of 
the process on Galaxy web port with no command prompt programming.  
 
The data for R1 and R2 was imported, and then I used barcode splitter to 
de-multiplex the amplicons after quality triming. (I did not use FASTQ groomer 
as Miseq data is supposed to be Sanger FastQ than Illumina). 
 
Then the sequence trimmer was used to trim the barcode+adaptor sequences. The 
results of this were re-uploaded and designated as FASTQ for alignment.
 
Now for the reference genome, as our aplicons are of from different sequences, 
we have segmented FASTA sequences in one file with different FASTA identifiers. 
When this file was input as the reference genome and mapping was performed 
using Bowtie for Illumina, the mapping went on with no errors. 
 
I could filter the alignment file using SAM filters too. But I can not do any 
more downstream visualozations, not even  SAM to BAM conversion. 
 
I suspect that this may be due to an error in the way that the reference genome 
was formulated but can not get around to figure it out. I would be extremely 
grateful if you could help me with this issue. I tihnk if I string together the 
sequences as one it would work, but converting this back for interpretation 
becomes an issue then.
 
Thank you,
Kind Regards,
Veranja 
Veranja Liyanapathirana
Graduate Student (Microbiology)
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