Hello Ines,

You have reached the support mailing list for the public Main Galaxy instance at http://usegalaxy.org. While we can help with many usage questions, sometimes for specific tools it is best to contact the tool author and/or lab running the instance hosting the tool. This is one of those cases.


I am guessing that you are using this tool from the Huttenhower Lab's public instance? http://huttenhower.sph.harvard.edu/galaxy/

Even though I suspect that you just need to assign the correct datatype in the UI, I honestly do not know enough about the tool implementation to confirm that as a solution, or help you with more details without going through the tool in more detail. The team there is certainly in a better position of experience to help. Their contact for questions and help with this tool is: https://groups.google.com/d/forum/picrust-users

Maybe you have already found this contact, but if not, hopefully it leads to a solution!

Best,

Jen
Galaxy team


On 1/29/14 1:46 PM, Ines wrote:
Hello,

I was trying to run a dataset through Picrust on the web-based Galaxy. I did the OTU table in Qiime as explained in http://picrust.github.com/picrust/tutorials/otu_picking.html#otu-picking-tutorial, with the command line: pick_closed_reference_otus.py -i $path/file.fasta -r $path/rep-set/97_otus.fasta -o $path/OTU_Table.biom.

I uploaded the file in Get Data, and I want to run the Normalize by Copy Number step, but I get the following message History does not include a dataset of the required format / build.

I am not sure if when I generate the OTU Table I need to specify a particular format, or what the problem is. I would really appreciate some advice.

I attach the biom file generated in case you need to take a look.

Thank you very much in advance.


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___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
using "reply all" in your mail client.  For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:

  http://lists.bx.psu.edu/listinfo/galaxy-dev

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