Hi Jennifer,
I reload my data with defined format type fastqsanger and it works now.
Thank you so much for your help.

Gang


On Thu, Feb 13, 2014 at 6:06 PM, Jennifer Jackson <j...@bx.psu.edu> wrote:

>  Hi Gang,
>
> I can't comment about a PC (I use a MAC), but this shouldn't matter. If
> you are using a local Galaxy (http://getgalaxy.org
> http://usegalaxy.org/toolshed), most tools will follow the same usage
> rules as when implemented on the public Main Galaxy instance at
> http://usegalaxy.org, the Galaxy CloudMan ami, and the bulk of other
> public Galaxy instances, at least for common tools.
>
> Exceptions would be if the tool(s) were specifically modified to function
> differently. Galaxy is open source and variations exist. The tool form of
> any alternate version (of a tool named the same) will likely note what
> modifications have been made. Plus you can always contact the other public
> instance hosts, if you have usage problems.
>
> Best!
>
> Jen
> Galaxy team
>
>
> On 2/13/14 2:26 PM, Gang Wang wrote:
>
>  By the way, can you use detect the fastq file using trimmer by column
> with your PC. Thanks a lot.
>
>
>  Gang
>
>
> On Thu, Feb 13, 2014 at 1:14 PM, Björn Grüning 
> <bjoern.gruen...@gmail.com>wrote:
>
>> Hi Wang,
>>
>> please check if your fastq file is associated with the correct fastq
>> format, fastqsanger probably.
>>
>> Cheers,
>> Bjoern
>>
>> > Hi,
>> >
>> > I just notice that NGS FASTQ Trimmer by column can't detect the fastq
>> > file I loaded. Anyone knows why. thanks a lot.
>> >
>> >
>> >
>> > On Fri, Jan 24, 2014 at 11:00 AM,
>> > <galaxy-user-requ...@lists.bx.psu.edu> wrote:
>> >         Send galaxy-user mailing list submissions to
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>> >         Today's Topics:
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>> >            1. Re: Creating a Trackster visualisation from a
>> >         reference in
>> >               your history (Jeremy Goecks)
>> >
>> >
>> >
>> ----------------------------------------------------------------------
>> >
>> >         Message: 1
>> >         Date: Thu, 23 Jan 2014 15:49:44 -0500
>> >         From: Jeremy Goecks <jgoe...@email.gwu.edu>
>> >         To: graham etherington (TSL)
>> >                 <graham.ethering...@sainsbury-laboratory.ac.uk>
>> >         Cc: galaxy-user@lists.bx.psu.edu
>> >         Subject: Re: [galaxy-user] Creating a Trackster visualisation
>> >         from a
>> >                 reference in your history
>> >         Message-ID:
>> >         <c3382f38-d243-4979-b6a7-ba13ef161...@email.gwu.edu>
>> >         Content-Type: text/plain; charset="windows-1252"
>> >
>> >         > Is it possible to create a custom build and use it to view a
>> >         SAM file without adding the .len and .2bit files in to the
>> >         Galaxy file system as an administrator?
>> >
>> >         Yes, it definitely is.
>> >
>> >         > If so, what am I doing wrong?
>> >
>> >
>> >         This is a Galaxy bug which has been fixed in this commit:
>> >
>> >
>> https://bitbucket.org/galaxy/galaxy-central/commits/117fef56513fc563dd231516196cfd601c1635e2
>> >
>> >         We have a release coming up, so this fix will be included in
>> >         the release and will make it to our public server soon. In the
>> >         meantime, note that you can use the genome fasta file rather
>> >         than the len file to create a custom build and everything
>> >         should work.
>> >
>> >         Thanks,
>> >         J.
>> >
>> >         -------------- next part --------------
>> >         An HTML attachment was scrubbed...
>> >         URL:
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>> >
>> >         ------------------------------
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>> >         End of galaxy-user Digest, Vol 91, Issue 21
>> >         *******************************************
>> >
>> >
>> >
>> > --
>> > Gang Wang
>> > Ph.D. student
>> > Veterinary Integrative Bioscience
>> > College of Veterinary Medicine & Biomedical Sciences
>> > Texas A&M University
>> > College Station, TX  77843-4458
>> >
>> >
>> >
>> >
>> >
>> >
>> >
>> > ___________________________________________________________
>> > The Galaxy User list should be used for the discussion of
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>>
>>
>
>
> --
> Gang Wang
> Ph.D. student
> Veterinary Integrative Bioscience
> College of Veterinary Medicine & Biomedical Sciences
> Texas A&M University
> College Station, TX  77843-4458
>
>
>
>
>
> ___________________________________________________________
> The Galaxy User list should be used for the discussion of
> Galaxy analysis and other features on the public server
> at usegalaxy.org.  Please keep all replies on the list by
> using "reply all" in your mail client.  For discussion of
> local Galaxy instances and the Galaxy source code, please
> use the Galaxy Development list:
>
>   http://lists.bx.psu.edu/listinfo/galaxy-dev
>
> To manage your subscriptions to this and other Galaxy lists,
> please use the interface at:
>
>   http://lists.bx.psu.edu/
>
> To search Galaxy mailing lists use the unified search at:
>
>   http://galaxyproject.org/search/mailinglists/
>
>
> --
> Jennifer Hillman-Jacksonhttp://galaxyproject.org
>
>


-- 
Gang Wang
Ph.D. student
Veterinary Integrative Bioscience
College of Veterinary Medicine & Biomedical Sciences
Texas A&M University
College Station, TX  77843-4458
___________________________________________________________
The Galaxy User list should be used for the discussion of
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To manage your subscriptions to this and other Galaxy lists,
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