*http://english.shanghaipasteur.cas.cn/Research2016/rp2016/202001/t20200125_229792.html*
*Wuhan coronavirus (2019-nCoV) evolution and human transmission
mechanism revealed by Institut Pasteur of Shanghai*
Date：25-01-2020 | 【Print
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The occurrence of concentrated pneumonia cases in Wuhan City, Hubei
Province of China was first reported on December 30, 2019 by the Wuhan
Municipal Health Commission.Thepneumonia cases were found to be linked
to a large seafood and animal marketin Wuhan. The Centers for Disease
Control and Prevention (CDC) and Chinese health authorities later
determined and announced that a novel coronavirus, denoted as Wuhan CoV
(nCoV-2019), had caused thepneumoniaoutbreak.The current public health
emergency partially resembles theemergence of the SARS outbreak in
southern China in 2002.Both happened in winter with initial cases linked
to exposure to live animals sold at animal markets, and both werecaused
by previously unknown coronaviruses.
The first genome sequence of the Wuhan CoV was released on Jan 10, 2020,
and subsequently multiple additionalWuhan CoVgenome sequences were
released. To understand the origin of the Wuhan CoV and its genetic
relationship with other coronaviruses, we performed phylogenetic
analysis on the collection of coronavirus sequences from various
sources. The results showed the Wuhan CoVs were clustered together in
the phylogenetic tree, which belong to theBetacoronavirus genera(Fig A).
It is likely that their natural host is bat. They and the SARS/SARS-like
coronaviruses shared a common ancestor that resembles the bat
coronavirus HKU9-1.
To investigate the Wuhan CoVand their host interaction, we looked into
the RBD domain of its spike protein (S-protein) of theWuhan CoV,
whichhad several patches of sequences with a high homology to that of
SARS-CoV_Tor2 and HP03-GZ01 (Fig B). The residues at positions 442, 472,
479, 487 and 491 in SARS-CoV S-protein were reported to be at receptor
complex interface and considered critical for cross-species and
human-to-human transmission of SARS-CoV. However, four of the five
critical residues are not preserved except Tyr491 in S-protein of
theWuhan CoV.
To assess the risk of human transmission of the WuhanCoV, we performed
structural modeling of its S-protein and evaluated its ability to
interact with human ACE2 molecules. The computational model of the Wuhan
CoV S-protein showed a Cα RMSD of 1.45 ？ on the RBD domain compared to
the SARS-CoV S-protein structure (Fig C). The binding free energy
between the Wuhan CoV S-protein and human ACE2 was -50.6 kcal/mol,
whereas that between SARS-CoV S-protein and ACE2 was -78.6 kcal/mol.Our
resultpoints to the important discovery that the RBD domain of the Wuhan
CoV S-protein supports strong interaction with human ACE2 molecules
despite its sequence diversity with SARS-CoV S-protein. Thus the Wuhan
CoV poses a significant public health risk for human transmission via
the S-protein - ACE2 binding pathway.
The research work entitled “Evolution of the novel coronavirus from the
ongoing Wuhan outbreak and modeling of its spike protein for risk of
human transmission” has published online in SCIENCE CHINA Life Sciences
on Jan 21st, 2020. Dr.Pei Hao(IPS, CAS), Dr.Wu Zhong (Beijing Institute
of Pharmacology and Toxicology), and Dr. Xuan Li (CAS Center for
Excellence in Molecular Plant Sciences, CAS) are co-correspondence
authors of this paper. Xintian Xu, Ping Chen, and Jingfang Wang are the
co-first authors.
The article “Evolution of the novel coronavirus from the ongoing Wuhan
outbreak and modeling of its spike protein for risk of human
transmission” giving evidence of the possibility of human transmission
of the current nCoV-2019 for public health risk was based on critical
sequence information by contributors listed below. We hope these joint
results would contribute to the common good of all people and all
parties involved.
On behalf of all authors:Xintian Xu^# , Ping Chen^# , Jingfang Wang^# ,
Jiannan Feng, Hui Zhou, Xuan Li*, Wu Zhong*, and Pei Hao*(^#
^Contributed equally to this work;*Corresponding authors)
We hope to thanks to:
For Accession ID: EPI_ISL_402120, EPI_ISL_402119, and EPI_ISL_402121
Originating lab and Submitting lab - National Institute for Viral
Disease Control and Prevention, China CDC
Authors - Wenjie Tan，Xiang Zhao，Wenling Wang，Xuejun Ma，Yongzhong
Jiang，Roujian Lu，Ji Wang，Weimin Zhou，Peihua Niu，Peipei Liu，Faxian
Zhan，Weifeng Shi，Baoying Huang，Jun Liu，Li Zhao，Yao Meng，Xiaozhou
He，Fei Ye，Na Zhu，Yang Li，Jing Chen，Wenbo Xu，George F. Gao，Guizhen Wu
For Accession ID: EPI_ISL_402124
Originating lab - Wuhan Jinyintan Hospital
Submitting lab - Wuhan Institute of Virology, Chinese Academy of Sciences
Authors -Peng Zhou, Xing-Lou Yang, Ding-Yu Zhang, Lei Zhang, Yan Zhu,
Hao-Rui Si, Zhengli Shi
For Accession ID: EPI_ISL_402123
Originating lab and Submitting lab - Institute of Pathogen Biology,
Chinese Academy of Medical Sciences & Peking Union Medical College
Authors - Lili Ren, Jianwei Wang, Qi Jin, Zichun Xiang, Yongjun Li,
Zhiqiang Wu, Chao Wu, Yiwei Liu
For Accession ID: EPI_ISL_402125 (MN908947|WH-Human_1)
Originating lab - unknown
Submitting lab - National Institute for Communicable Disease Control and
Prevention (ICDC) Chinese Center for Disease Control and Prevention
(China CDC)
Authors - Zhang,Y.-Z., Wu,F., Chen,Y.-M., Pei,Y.-Y., Xu,L., Wang,W.,
Zhao,S., Yu,B., Hu,Y., Tao,Z.-W., Song,Z.-G., Tian,J.-H., Zhang,Y.-L.,
Liu,Y., Zheng,J.-J., Dai,F.-H., Wang,Q.-M., She,J.-L. and Zhu,T.-Y.
Full text
link:http://engine.scichina.com/publisher/scp/journal/SCLS/doi/10.1007/s11427-020-1637-5
