Hello,

The dark bar is an exon - this display is an indication that there is a base 
level alignment at this position between the EST and the genome. The rest of 
the data (other sequences) appears to support the idea that this is instead an 
intronic region (no mRna/Est alignments at this location, but at locations at 
one or either edge). 

Zoom out a few levels to see the big picture. Then try 10x to see where the 
other sequence have exons.

We hope this helps,
Jennifer

------------------------------------------------ 
Jennifer Jackson 
UCSC Genome Bioinformatics Group 

----- "Chuangye" <[email protected]> wrote:

> From: "Chuangye" <[email protected]>
> To: [email protected]
> Sent: Monday, November 9, 2009 4:14:28 AM GMT -08:00 US/Canada Pacific
> Subject: [Genome] What differences between these ests
>
> Dear sir,
> 
> Thank you for reading my Email!
> 
> First, please set "chr3:173,402,247-173,402,431" in your
> "position/search"
> of UCSC Browser. Then set "full" of "Human EST" in "mRNA and EST
> Tracks".
> And "Refresh". OK, now you will see the picture of the attached
> figure. As
> you will see, a few ESTs with thin lines plus arrows but only one with
> thick
> line at the bottom with no arrows as upper ESTs. OK. My question is
> what the
> differences between the last one
> (EL584176<http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=nucest&term=EL584176&doptcmdl=GenBank&tool=genome.ucsc.edu>)
> with others ?
> 
> Thank you!
> 
> Best wishes,
> 
> Chuangye
> 
> _______________________________________________
> Genome maillist  -  [email protected]
> https://lists.soe.ucsc.edu/mailman/listinfo/genome
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